The Campeare of the Effects Progesterone and Calcium Lonophore (A23187) on Mouse Sperm Parameters and Fertilization Rate

Purpose: The aim of this study to investigate the effect of progestrone and calcium ionophore (A23187) on mouse sperm parameters and fertilization rate. Materials and Methods: Csauda epididymidis were then obtained from NMRI male mice (10-12 wk old) and were divided to three groups: Control, adding progesterone (10µg/ml) and addingionophore (10mM) After sperm were prepared and incuhated in T6 medium in an atmosphere of 95% air, 5% Co2 and 37˚C sperm parameters were analized as WHO.The oocytes were also ohtained the same strain of female NRMI mice (7-10 wk old) and inseminated with sperm. Then rate of fertilization and embryo development were cheked daily during 120h culture period. Finally the result of each groups were analyzed and comparied by ANOVA, X2 and exact fishers test. Results: The results indicated that the main of grade three of sperm movement in progesterone group was increased significantly (P<0.05) compared with control groups, were as the value was not significantly different in calcium ionophore compare to control. The number of embryos derived from sperms that treated with calcium nophore significantly were higher than two others groups (P<0.05). Conclusion: The progesterone and calcium ionophore can increased the sperm motility, and fertilization rate in mouse.